Today I used the McFarland standards to find the CFU/ml of Francisella tularensis LVS that corresponds to each number.
I used the 0.5, 1, and 2 standards to create 2mL solutions of LVS that matched the visual optical density. The solutions were then serial diluted 1:10 to -7 and plated on CHOC II plates using the spot plate method (five 10microliter spots for each dilution in a quadrant of the plate).
The plates will be incubated at 37C for 48 hours until CFU can be counted.
//BEE
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