This blog is the ONS (Open Notebook Science) record for the work that I personally perform in the lab. It is posted informally and without peer review. Please feel free to comment and contact me at bridget.eklund@ndsu.edu if there is something you're interested in. You can learn more about the lab on our wiki page (http://openwetware.org/wiki/Fisher). Thanks for visiting.

Tuesday, October 21, 2014

Francisella LD50 in Blaptica dubia roaches

from 10/17/14

Unfortunately due to complications with the media, spot titer plates did not produce individual colonies to determine CFU/dose for the three Francisella strains being tested. Because of this, the LD50 cannot be determined.
Francisella plates

However, the spot plates for Burkholderia K56-2 strain and E.coli DH5alpha were able to be read, and the PBS control showed no signs of bacterial contamination.
E.coli, K56-2, and PBS plates


Roaches in 37 deg C incubator
The roaches are being tracked for survival and will be observed for 6 more days.











//BEE 
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