LD50 of Francisella in Wax Worms

In addition to finding the LD50 values Francisella mutants in the Dubia roaches, I have also been repeating the work done by Ben to find the LD50s in the Greater Wax moth. I used the same protocol that was used for finding the LD50s of Listeria for Dr. Bergholz (Listeria LD50 protocol using wax worms).

A few modifications were made to accommodate for the fastidious nature of Francisella, which will not grow well in broth cultures. Mueller-Hinton plates with 2% Isovitalex were used to make 48 hour plates of each Ft culture. A loop of bacteria was suspended in 1ml of sterile PBS to form a bacterial suspension, which was then diluted with PBS.

//BEE